The corresponding primer sequence used for each reaction.The original trace file (.ab1 file) provided by your sequencing company or core facility.If you obtain a different sequence than expected and wish to contact Addgene about the accuracy of your plasmid, please email and include: This is an example of a trace file from a high-quality portion of a sequencing reaction: You should receive your sequencing results as a trace file (.ab1) which graphically depicts the sequence as a series of colored peaks corresponding to one of the four nucleotide bases. If you require additional sequencing and need to design a custom primer, Addgene recommends using Addgene's sequencing results as a reference for primer design.Ī good sequencing reaction will produce between 300-900 base pairs of useable sequence. Is your custom primer found within the Addgene sequencing results? What primers were used by Addgene during quality control?Īddgene lists the primers used to obtain each result above the posted sequence in the "View Sequence" link. Many sequencing cores have a list of common primers that can be requested for no additional charge. There are many widely used, common primers that are often found within the plasmid backbone (See Addgene's Sequencing Primers for reference). The primer should be a minimum of 50 nucleotides and a maximum of 300 nucleotides from your target.Īre there commercially available primers that can be used for sequencing? Is the primer an appropriate distance from the target? Make sure the primer only anneals once within the entire construct. He’s also a fan of board games and spending time with family.There are a number of factors to consider when selecting sequencing primers, including: Porto looks forward to biking, hiking, and traveling in Arizona. ![]() He has worked at university hospitals, Veterans Administration, and the Epilepsy Foundation.ĭr. He returned to San Antonio for his fellowship training in clinical neurophysiology. He completed an internship in internal medicine at the University of Kansas Medical Center then a residency in neurology at the University of California Irvine Medical Center. Porto received his medical degree from the University of Texas Health Science Center at San Antonio. He has also worked extensively evaluating and treating dementia, strokes, seizures, tremors, Parkinson’s disease, multiple sclerosis, neuropathy, traumatic brain disease, and vertigo.ĭr. He comes to us from San Antonio, Texas where he was in private practice. Mariano Porto is joining our team of neurologists. Mariano Porto Joins Four Peaksįour Peaks Neurology is proud to announce Dr. If you have further questions, please feel free to contact our office. Please review the information that we have provided for you. The page contains important information as to what you need to bring, including forms to download, print, fill-out and bring with you to your first appointment. We kindly ask that all of our new patients visit our First Time Visit To Our Practice page in advance of your first appointment. Medical Records Release Forms are available for this purpose. Please read the Uses and Disclosures of Protected Health Information, Notice of Privacy Practices (HIPPA) that our office uses to describe how medical information about you may be used and disclosed and how you can obtain access to this information.Īs with all medical practices, it may occasionally be necessary to share your medical information with other treating physicians and/or health care affiliates as may be needed. We respect your privacy and will keep your information confidential. ![]() This information is designed to help you understand that your health care with our practice is very important to us and we want to make sure that you, our patient, understand that there are procedures that must be followed to provide you with quality care in a timely and efficient manner. ![]() ![]() If you are new to our practice, please review our Office Policy and Procedure sheet. You will find copies of our office forms available for download, which can be filled out at your convenience, prior to your first visit. Please take the time to review all of the information that is available to you. We strive to provide the best possible service and treatment available to our patients. We hope the information you find here will help you become familiar with our Physicians, our practice, and the medical services we offer.
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