The corresponding primer sequence used for each reaction.The original trace file (.ab1 file) provided by your sequencing company or core facility.If you obtain a different sequence than expected and wish to contact Addgene about the accuracy of your plasmid, please email and include: This is an example of a trace file from a high-quality portion of a sequencing reaction: You should receive your sequencing results as a trace file (.ab1) which graphically depicts the sequence as a series of colored peaks corresponding to one of the four nucleotide bases. If you require additional sequencing and need to design a custom primer, Addgene recommends using Addgene's sequencing results as a reference for primer design.Ī good sequencing reaction will produce between 300-900 base pairs of useable sequence. Is your custom primer found within the Addgene sequencing results? What primers were used by Addgene during quality control?Īddgene lists the primers used to obtain each result above the posted sequence in the "View Sequence" link. Many sequencing cores have a list of common primers that can be requested for no additional charge. There are many widely used, common primers that are often found within the plasmid backbone (See Addgene's Sequencing Primers for reference). The primer should be a minimum of 50 nucleotides and a maximum of 300 nucleotides from your target.Īre there commercially available primers that can be used for sequencing? Is the primer an appropriate distance from the target? Make sure the primer only anneals once within the entire construct. He’s also a fan of board games and spending time with family.There are a number of factors to consider when selecting sequencing primers, including: Porto looks forward to biking, hiking, and traveling in Arizona. ![]() He has worked at university hospitals, Veterans Administration, and the Epilepsy Foundation.ĭr. He returned to San Antonio for his fellowship training in clinical neurophysiology. He completed an internship in internal medicine at the University of Kansas Medical Center then a residency in neurology at the University of California Irvine Medical Center. Porto received his medical degree from the University of Texas Health Science Center at San Antonio. He has also worked extensively evaluating and treating dementia, strokes, seizures, tremors, Parkinson’s disease, multiple sclerosis, neuropathy, traumatic brain disease, and vertigo.ĭr. He comes to us from San Antonio, Texas where he was in private practice. Mariano Porto is joining our team of neurologists. Mariano Porto Joins Four Peaksįour Peaks Neurology is proud to announce Dr. If you have further questions, please feel free to contact our office. Please review the information that we have provided for you. The page contains important information as to what you need to bring, including forms to download, print, fill-out and bring with you to your first appointment. We kindly ask that all of our new patients visit our First Time Visit To Our Practice page in advance of your first appointment. Medical Records Release Forms are available for this purpose. Please read the Uses and Disclosures of Protected Health Information, Notice of Privacy Practices (HIPPA) that our office uses to describe how medical information about you may be used and disclosed and how you can obtain access to this information.Īs with all medical practices, it may occasionally be necessary to share your medical information with other treating physicians and/or health care affiliates as may be needed. We respect your privacy and will keep your information confidential. ![]() This information is designed to help you understand that your health care with our practice is very important to us and we want to make sure that you, our patient, understand that there are procedures that must be followed to provide you with quality care in a timely and efficient manner. ![]() ![]() If you are new to our practice, please review our Office Policy and Procedure sheet. You will find copies of our office forms available for download, which can be filled out at your convenience, prior to your first visit. Please take the time to review all of the information that is available to you. We strive to provide the best possible service and treatment available to our patients. We hope the information you find here will help you become familiar with our Physicians, our practice, and the medical services we offer.
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![]() ![]() In fact, it may be easier to set up on a Windows PC. Here's the catch: you need extra software to initially set it up (Apple AirPort Utility), and if you're doing so on a Mac you'll actually need to download a modified, *older* version of AirPort Utility, as the latest versions completely remove support for these old guys. You can buy a Raspberry Pi and SD card and set them up to do the same thing, but it's going to take a lot of effort and time to initially set up (and you better be familiar with Linux o r willing to learn!), or you can buy an outdated AirPort Express for a fraction of the price, set it up, and play music through it. This is the device that introduced AirTunes (which became AirPlay when it was expanded to the Apple TV and video support was added), and it remains very useful as a digital audio streaming target. IPhones and iPod touch devices, as well as Mac computers, or iTunes on a Windows PC can all stream audio to Apple AirPlay- and AirTunes-certified devices. However, this thing is extremely useful for one purpose: wireless music streaming from an Apple device. ![]() ![]() This product is outdated at this point, and the wireless router/cable modem combination I get from Time Warner Cable is much more capable than this thing is. This is the cheapest way to stream music from an iPhone or iPod, but it's a bit of a pain to configure at first. Perhaps it’s because I had a beta version of iTunes, who knows. Of course it wasn’t that easy in my case, it kept failing at the “Restoring in iTunes” step. It’s called SeasOnPass, it’s free, just follow the official instructions and you’ll be fine. There are various options for jailbreaking but since we’re going to install aTV Flash as our operating system, let’s use the jailbreak software that they’ve built themselves.
![]() Focusing on the strict definition of autonomy and failing to recognize an individual as part of a family leads to an incomplete understanding of decision making for informed consent. It is both common and socially acceptable for a patient to consult family members in order to aid in decision making. Agency, however, allows a patient to consider how a decision he or she makes might affect his or her family members. Reminders to respect a patient’s liberty are generally directed toward clinicians, but family members can certainly coerce, persuade, or manipulate a patient. Respecting patient autonomy thus includes respecting both how patients wish to make a decision and the decision made, even if the decision is to allow their family’s desires to supersede their own. Patients often wish to take their family members’ opinions into account when making medical decisions, as they would with many other important decisions. ![]() Although this emphasis is intended to prevent patients from being coerced, especially by medical professionals, it ignores that a patient is part of a family. American bioethics circumscribes the role of others who might influence the patient to make a choice that does not put his own wishes or best interests first. It requires physicians to respect patients’ autonomy by giving them the information needed to understand the risks and benefits of a proposed intervention, as well as the reasonable alternatives (including no intervention), so that they may make independent decisions.Ī patient’s independence is traditionally the highest priority in American bioethics. The ideal of “informed consent” is a hallmark of Western medical ethics that came about following the horrors recounted in the Nuremberg trials and was codified in American law through Canterbury v. Beauchamp and Childress remind us that autonomy requires both “liberty (independence from controlling influences) and agency (capacity for intentional action)” and that liberty is undermined by coercion, persuasion, and manipulation. So, we might wonder at this point, what is the appropriate nature and scope of autonomy and the role of family members in a case like Dave’s? Patient Autonomy and Informed ConsentĮxpressing respect for patients’ autonomy means acknowledging that patients who have decision-making capacity have the right to make decisions regarding their care, even when their decisions contradict their clinicians’ recommendations. Barelle is in a challenging position as she is newly involved in Dave’s care and does not have a long-standing relationship to serve as a foundation for these difficult conversations. She must also remain an advocate for his stated wishes without being paternalistic about his choice to follow his family’s wishes. Barelle must consider how to respect both his autonomy and the role of his well-intentioned and loving family in his care. What should she do? Should she downplay the potential of aggressive care to better persuade the family to respect the patient’s wishes? Commentary Barelle has suspected that Dave’s wishes were diverging from his family’s, and she wonders how to acknowledge this and how to advocate on Dave’s behalf in ways that won’t be divisive. He finally admits that aggressive acute care seems to him to be excessive and futile at this point but that he doesn’t want to let his family down by not “fighting.” He fears that his family thinks of hospice and palliative care as capitulating and “giving up.”ĭr. Barelle continues to describe what aggressive acute care would entail over the next months and begins to probe deeper into Dave’s goals of care, Dave slowly confesses that he worries about aggressive treatment, states that it isn’t what he wants, and also says, “I’m not the hero type.” He goes on to express that he particularly values his independence and that he fears pain, suffering, impending loss of functioning, and loss of his autonomy. Dave insists on pursuing aggressive acute care for his prostate cancer, but he also seems exhausted. Barelle, sits down alone with Dave to discuss his end-of-life wishes. Dave’s physicians, however, now want to transition his care and incorporate hospice and palliative approaches to managing his illness. Dave’s family has remained optimistic, confident, and encouraging they fully expected him to pursue aggressive treatment. When he’s been in and out of the hospital for four months or visiting clinic for lab tests and treatments, he’s often accompanied by his wife Jessica and one or both of his two children, Christine and Alex. Despite surgery, chemotherapy, radiation, and hormonal treatments, his prostate cancer has metastasized to his bones. ![]() South of the lakes, the current changes with the tide at Suez. In general, the water in the canal north of the Bitter Lakes flows north in winter and south in summer. It contained, according to Alois Negrelli's plans, no lock systems, with seawater flowing freely through it. The original canal featured a single-lane waterway with passing locations in the Ballah Bypass and the Great Bitter Lake. In 2020, more than 18,500 vessels traversed the canal (an average of 51.5 per day). Its length is 193.30 km (120.11 mi) including its northern and southern access-channels. The canal extends from the northern terminus of Port Said to the southern terminus of Port Tewfik at the city of Suez. ![]() It offers vessels a direct route between the North Atlantic and northern Indian oceans via the Mediterranean Sea and the Red Sea, avoiding the South Atlantic and southern Indian oceans and reducing the journey distance from the Arabian Sea to London by approximately 8,900 kilometres (5,500 mi), or 10 days at 20 knots (37 km/h 23 mph) to 8 days at 24 knots (44 km/h 28 mph). The canal officially opened on 17 November 1869. Construction of the canal lasted from 1859 to 1869. In 1858, Ferdinand de Lesseps formed the Suez Canal Company for the express purpose of building the canal. The canal is a route of trade between Europe and Asia. The Suez Canal ( Egyptian Arabic: قَنَاةُ ٱلسُّوَيْسِ, Canāt el Sewes) is an artificial sea-level waterway in Egypt, connecting the Mediterranean Sea to the Red Sea through the Isthmus of Suez and dividing Africa and Asia. ![]() What surprised me the most is that the team is able to build a rapport, they made me communicate so easily and I was able to share my bucket list with them with no hesitation. They are aware of what needs to be done, they are always available to help, they are flexible and most importantly they support and work with you to reach your goal. All instructors of my health team including Yoga, Meditation and Nutrition are very well educated and have deep knowledge. I am getting good concentration towards Yoga and building my regular practice. Within the short span of FlexifyMe I have started to gain knowledge about Yoga and most importantly to know my body & mind better than I used to. I had heard about Yoga and wanted to know about authentic Yoga & meditation with a focus to better health and mind. ‘’Due to the sedentary lifestyle & long driving hours everyday I had developed back & joint pain. I congratulate the team and wish them success and again highly recommend this to anyone ’’ It has become from nothing to almost an addiction in all good ways. The icing on the cake was with my trainer who has been so kind and I look forward to my sessions more than anything else I would do during the day. After attending the first free session and getting the detailed analysis was totally surprising for me with the specificity around personalized wellbeing and ask. I would also like to add that being a total non-believer of Yoga practices and having never paid attention to this coming from India and even with a highly religious and spiritual background Yoga and meditation had eluded me till date. I would definitely recommend FlexifyMe to anyone who is seeking such goals and has those desires and outcomes. I love the model and would recommend it more online and self service. I am super pleased with the value I got at this cost and it couldn’t have been better than this. That made me very satisfied and happy about the choice I made months ago. The team works well and in coordination always to address any questions or concerns that I had I was really surprised that there can be such an effective online way of doing this. I have received great service, acknowledgement, concern and responsiveness from everyone at the company. I have been extremely pleased and satisfied with FlexifyMe. ![]() ‘’I have a very busy schedule and my goal was to be positive, peaceful and relaxed & to maintain balance in my life. I love how my yoga guru weaves in back exercises in our sessions so seamlessly that only later on I realize that I exercised my back very well along with lots of other asanas for the rest of the body as well as mind. There have been days when I feel absolutely zero pain. I realized that just a small change in my standing posture can have such a big impact on my lower spine. I love the personal attention, accountability, convenience of timing & having it all available from home. ![]() I definitely feel really good after each Yoga session & have increased peace of mind that I am on track of caring for myself. I have started to incorporate many elements of my new vedic nutrition plan by a nutritionist & learning a lot from my Yoga trainer. It's been around three months and I'm loving it so far. I am really happy with my decision to join FlexifyMe. ![]() After doing a demo session with FlexifyMe and the idea of a personal health team to achieve my goals sounded ideal. If you are going to purchase, don't expect miracles, or even its own specifications to be close to accurate.‘’I have been wanting to set guidelines for my diet, including Yoga for general health & also to work on my lower back pain for a very long time but couldn't do so due to the hectic life. I have considered directional antennas, but I think these will be tossed in the box labelled of "crap for another day" The video output signal voltage does appear to run slightly high over a 75R resistor load which also explains why an older monitor I originally used for testing wouldn't work, Lucky for me the cheaper generic monitor I have with built in buffer resolved this problem. But line of sight (LOS) struggled to maintain signal at 450mt. And the transmitters run extremely warm - perhaps I could fit aftermarket cooling or heat sink if I found a line of sight application less than 400mtĪs in the description states "2000mt range" I was hopping for half of this considering the price being to good to be true. Both transmitters are working on regulated 12VDC with less than 100mV drift. same issues with both and interchanging the pair.Īnd yes they work for very short range. You can check your FICO® Score for free on our free mobile app, UFirst. Your bank routing number is the first nine-digit number on the bottom left corner of each check. Reversely, if a borrower finds themselves with a credit score in the mid-600s or low 500s to low 600s, they may expect a lender to give an interest rate three to five times higher (between 10–24%) than those with good or excellent credit. Where are my account and routing numbers on a check. The routing number, or ABA, for America First Credit Union is 324377516. Some banks and credit unions make it difficult to find, so we’re here to make it fast and easy. What Are America First Routing Numbers Used For. It can be found on the checks, and sometimes the bank or credit union has it on their website. It is also referred to as an RTN, a routing transit number or an ABA routing number and can be easily be found printed on the bottom left side of your check. Who needs the America First Credit Union Routing number Anyone who banks at America First Credit Union might need to find the routing number. Its based on the location of the bank where your account was opened. ![]() Typically, to get an interest rate between 0–2% on new car loans, the lender will require a credit score of 700 or higher. Your bank routing number is a 9-digit code used to identify a financial institution in a transaction. Routing Number: 103105637: Institution Name: FIRST AMERICAN BK : Office Type: Main office: Delivery Address: P.O. ![]() This credit score also determines the borrower’s interest rate on the loan. The routing number 103105637 is assigned to FIRST AMERICAN BK. When getting approved for an auto loan, most financial institutions will look at the borrower’s industry-specific auto FICO® Score3, along with their base FICO® Score from the three consumer credit reporting bureaus: Equifax, Experian and TransUnion. On average, the borrower needs a score in the low 700s for a new car loan and mid-600s for a used car loan.Ī borrower's credit history tells the lender whether or not they are high or low risk. As indicated above the routing number for a checking account and savings account for a specific bank location will be the same. ![]() The credit score required to qualify for an auto loan is relative to the lender. Speech Access - Transfer from your Share Savings or Checking to a loan within your account or to another America First account through our automated phone system. Routing Number 63100277 is the Bank of America Routing Number Florida However if you opened your checking or savings account in a different state then it is possible that your routing number will different. Aurora Snow directed several films for Sin City/Mayhem including Assploitations 1-5 (2003-2005), Anal Delinquents 1-3 (2004-2005), Perverted Tales (2004), Sweet Obscenities (2004), Twist of Anal (2005), Teen Chocoholics (2005) and Back 2 Black (2005).Īurora at 2005 AVN Awards – Adult Empire GalleriesĪ turning point in Aurora Snow’s adult film career came in 2003 when she was named “Female Performer of the Year” at the AVN Awards. Collaborating with this prominent production company enabled Aurora Snow to make her directorial debut with the Sin City production Assploitations (2003). I discovered the free spirit within, embraced the experience, and marked the beginning of a very unexpected career”.īetween 20 Aurora Snow was a contract performer for Sin City. I had been a shy, conservative girl with more book smarts than street smarts. A thespian at heart, I enjoyed the performances more than I expected, and soon shed the promise I made to myself to leave the industry within a year. They dressed me in school-girl uniforms and tied my blonde hair into pigtails-anything to make me look every bit as young as I was. I quickly adjusted to playing the part of nymph. I was put in movies with titles like Naughty Little Nymphos and Trained Teens. Producers clamored to get me into their films. Because, as I quickly learned, when you work in the porn industry, “barely legal” is synonymous with “VIP.” Directors lavished attention upon me. ![]() Those first couple of years, I was in demand-a new face, fresh, hot, and most importantly, young. ![]() Two scenes in one day was my introduction to the world of porn, and for the next few years, it was the type of routine that I would come to see as perfectly normal… I drove over to his house right away and shot my first porn scene of many-and directly after that, drove to my second “first” scene with the director who’d originally scheduled me. He offered me an outrageous sum of money if only he could have my first scene. “Hours before I was to do my first video shoot, I received a call from Ed Powers, a well-known producer of amateur girls performing for their first awkward time on camera. Talking about shooting her adult film industry debut in her August 2010 Daily Beast article, Aurora Snow revealed how My career in porn started out with a few photo shoots, but since my goal was to make as much money as possible in one year and then get out, run, and never look back, I decided to start doing video the very next week”.Īurora at 2003 AVN Expo Aurora Snow : Adult Film CareerĪurora Snow first began working in the adult film industry in May 2000 and one of her earliest porn performances was featured in the Ed Powers sequel release More Dirty Debutantes 152. That was how I ended up in the offices of Jim South, who had also notoriously recruited the underage Traci Lords… In search of a high-paying job that required very little of my time, I found a newspaper ad that read “Girls make 2,000/day.” In much smaller print under that it read “nude modeling,” and then listed a phone number. “I was 18 years old-“ barely legal,” in industry jargon-and halfway through my first year of college at UC Irvine, worried about the debt I was incurring. As Aurora Snow herself explained within her Daily Beast article Wondering how Aurora first discovered the adult industry? It all began when she was 18 years old and responded to a modelling ad in a newspaper. Aurora Snow graduated from high school when she was 17 whereupon she enrolled at UC Irvine majoring in theatre but ultimately switching to business. Hailing from Santa Maria, California, Aurora Snow was an honor roll student who had started performing in community and regional theatre productions when she was 12 years old. Now a freelance journalist, producer and actress, Aurora Snow is widely regarded as one of the adult entertainment industry’s most popular crossover stars. During this time Aurora Snow was named “Female Performer of the Year” at the 2003 AVN Awards and she has since been inducted into the “Hall Of Fame” at the AVN and XRCO Awards. ![]() Number of Scenes : 500+ (as a performer), 14+ (as a director)Īurora Snow is a Daily Beast writer who used to worked in the adult film industry as a performer, model and director for over 10 years. Tattoos : Black cat on the back of her neck Aurora Snow Twitter : Daily Beast Author Profile : Aurora SnowĪurora at 2011 XRCO Awards, Image Attribution : © Glenn Francis, Aurora Snow – Personal Info ![]() ![]() Reward: What the main long-term reward of the challenge is (ignoring clones/chocolate/GP/etc.).Ĭap: How many of these can be done while increasing the main reward. Note that I skipped UAC (see UBC effectively) and the Day challenges – as you can always just repeat them if you think you can do significantly better than the last time, and they don’t take long enough to bother scheduling them, unlike challenge series that will often take weeks or months to complete. Pets: MAG Balrog 5.978 E+9 HP, SUP Santa 3.667 E+9 HP, ASS Shark 4.327 E+9 HP, MAG Witch 2.549 E+9 HP, ROG Squirrel 3.674 E+9 HP, DEF Whale 12.Each challenge section will have the following information. ROG Squirrel did a total damage of 6.302 E+12 and received 319.250 E+9 damage.ĭEF Whale did a total damage of 0 and received 1.079 E+12 damage. MAG Witch did a total damage of 82.346 E+12 and received 221.459 E+9 damage. SUP Santa also healed 2.843 E+12 HP.ĪSS Shark did a total damage of 83.827 E+12 and received 387.554 E+9 damage. ![]() SUP Santa did a total damage of 2.422 E+12 and received 318.647 E+9 damage. MAG Balrog did a total damage of 81.550 E+12 and received 535.434 E+9 damage. Your free exp pool gained 2.012 E+6 (980,625 from patreons) experience.Ĭongratulations, you defeated evolved Balrog! ROG Squirrel gained a total of 10.322 E+6 experience.ĭEF Whale gained a total of 10.322 E+6 experience. MAG Witch gained a total of 10.322 E+6 experience. SUP Santa gained a total of 10.322 E+6 experience.ĪSS Shark gained a total of 10.322 E+6 experience. MAG Balrog gained a total of 10.322 E+6 experience. Here is the Data Dump from a 3-10 run (with scripts running)ĭungeon result for Volcano 3 (5/5/10), 2185 rooms They each have a Fire Weapon and Armour/Accessory of their element.Īll my dungeoning Teams have the Same Element front row with Countering Element back row, one for each element dungeon And a spread of Classes. ![]() I've evolved 93 of the 94 pets and equipped all of my Dungeoning pets. I have gear and gems equipped to all my pets so maybe that makes a difference, I haven't looked into whether Challenge completions or Static Multipliers have any effects. Free Lucky Draws in Ads (You get 100 AdPoints)ĭid you master the highest difficult in the dungeons? My Pets are Level 500+ and with the best Equip still has no chance in Volcano 3-10 The short answer is yes, I can beat every dungeon on Depth 3, Difficulty 10 now. Rising Baal Power for buying Light Clones Unlock Buy Creation (important for some Challanges) Unfortunately the pets don't find any items I found a solution but the result is not as it should be. I found a way, but one Pet goes in negative Exp, so i have now 6 Pets with Level 700 and one with 2 billion negative exp but still level 1ģ) The Infinity Tower is also hard to hack. Having to spend literal weeks in Infinity at this point is a massive roadblock, and it prevents quick farming of T4 crafting mats, and a 6th Pet Teamġ) The Exp Multiplier is harder to find then i thought. I just really want to see and feel the power of a level 200 pet team.Ĭrafting Tier 3 or 4 gear is very slow, and it sucks when you're missing a few Mithril and need to wait. (Free EXP could be similar to how you did Creation Costs and it being a negative figure) Pet EXP Multiplier, either direct multiplying when Pets perform actions or the Free EXP Pool being multiplied. For consideration, is it possible to add: These modes may be activated by HPGL command in the sign making software or from the Allen Remote Panel program.Ī wide variety of vinyls, films, papers, stencil, sandblast mask and banner materials can be handled by the 230 for cutting, drawing, plotting and banner making. ![]() The 230/315 Vinyl Cutter has two modes of operation: Cut and Draw. ![]() High has a top force of 550 grams, and is used when cutting heavier materials such as sandblast mask, magnetic sheeting, etc. Low is sufficient for most vinyl cutting applications, and has a top cutting pressure of 250 grams at 100% on the cutter's force dial. The two modes are listed as FORCE POT RANGE, high and low. These modes are contained in the OPTIONS menu under SETUP in the remote panel program. There are 2 modes to the force adjustment, which can be accessed through the Windows remote panel provided with the cutter. The Model 230 features Allen's exclusive Gold Touch Cutting head, which allows the user up to 550 grams of cutting pressure. The Allen Remote Panel program provides direct user control of speed, force and position, as well as having a full gamut of diagnostics. The 230/315 uses HPGL command language for widespread compatibility and emulates the Roland CAMM-1, the Hewlett Packard 758x, and DMPL plotters. Most of the operational procedures are the same for both the 230 and 315 cutters, with the exception of the differences relating to the material transport method, and the ability of the 315 to produce pounce patterns with the optional pounce kit available from Allen Datagraph Systems.Ī Model 315 addendum appears at the end of this manual, which outlines the different operational procedures relating specifically to the 315.Ī thumb screw tool holder allows tools to be quickly and easily changed. The Model 315 utilizes precision sprocket assemblies which accept the standard IBM compatible punch pattern as well as "universal punch" vinyls and Gerber 15" punched vinyl. The 230 utilizes a precision grit wheel system for positive tracking and movable pinch wheels for handling punched or unpunched materials in standard and nonstandard sizes. The 230/315 cuts with a swivel knife or draws with various pens and markers. This item can be shipped worldwide.The Allen Datagraph Systems Model 230 and 315 Vinyl Cutters are high performance machines designed for the graphics professional to cut and draw on a wide variety of materials. The seller is “nevadasurplus-com” and is located in Sparks, Nevada. This item is in the category “Business |
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